Figure 5.

Addition of IL-21 during feeder expansion results in T cells with a less-differentiated phenotype. Phenotype of in vitro differentiated T cells after polyclonal feeder expansion, gated on eGFP⁺ transduced cells, or eGFP⁺ CD62L⁺ cells for (b). Cytokine(s) as shown was/were added during both agonist selection and feeder culture. Percentage of CD62L⁺ cells (a) and CD62L MFI (b). Individual values and mean ± SD of 4 donors. Results were compared using Kruskal–Wallis test followed by Dunn’s multiple comparisons test. p-value < 0.05 (*), p-value < 0.01 (**). (c) Percentage of cells in CD45RA/CD45RO quadrants of dotplots. Mean ± SD of 4 donors. Results were compared using two-way ANOVA followed by Dunnett’s multiple comparisons test. CD45RO⁺RA⁻ p-value < 0.05 (*) for IL-21 vs IL-7 + 15 and p-value 0.01 (**) for IL-21 vs IL-15 + 21. (d) Percentages of T cell subsets. T(N), naive T cells (CD45RA⁺ CD62L⁺ CXCR3⁻ CD95⁻); T(SCM), stem cell memory T cells (CD45RA⁺ CD62L⁺ CXCR3⁺ CD95⁺); T(CM), central memory T cells (CD45RA⁻ CD62L⁺ CXCR3⁺ CD95⁺); T(EM), effector memory T cells (CD45RA⁻ CD62L⁻ CXCR3⁻ CD95⁺); T(EFF), effector T cells (CD45RA⁺ CD62L⁻ CXCR3⁻ CD95⁺). Results from a representative co-culture