Figure 8.

Polyclonal expansion of in vitro differentiated T cells in the presence of IL-21 leads to superior IFN-γ production. Intracellular staining of in vitro generated T cells for IFN-γ, after co-culture of in vitro differentiated T cells with T2 cells pulsed with relevant (WT1) or irrelevant (INF) peptide (10 µg/ml), THP-1 or HL-60-A2 (both HLA-A2⁺ WT1⁺), or JY (HLA-A2⁺ WT1⁻) cells. Culture medium was used as a negative control (neg ctrl), stimulation with aCD3/aCD28 as a positive control (pos ctrl). Effector/target ratio 1/2. Gating on eGFP⁺ TCR-transduced cells. Cytokine(s) as shown was/were added during both agonist selection and feeder culture. Individual values and mean ± SD of 4 donors, unless otherwise indicated. Results were compared using two-way ANOVA followed by Tukey’s multiple comparisons test. p-value < 0.05 (*), p-value < 0.01 (**), p-value < 0.0001 (****). IL-21 and IL-15 + IL-21 were significantly different from the other cytokine conditions, not from each other