Figure 2.

Hypoxia induces HIF-1α and HIF-1-target genes expression in CAFs. (a) CAFs cultured in hypoxia (1% pO2) for 48 hrs express more HIF-1α (but not HIF-2) compared to their normoxic counterpart. The HIF-1α/Actin ratio was calculated by densitometry and normalized to “1” for each normoxic CAF (A.U: arbitrary units). (b) Expression of HIF-1α target genes (VEGF, CA-IX, GLUT1, PDK1), tested by RT-qPCR, is increased in hypoxic CAFs. Results are expressed as the mean ± s.d. of three independent experiments, normalized to “1” in normoxic conditions (N: Normoxia; H: Hypoxia). P values were determined by unpaired two-tailed student’s t-test. (*p < .05; ** p < .01)