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. 2021 Apr 2;1(1):100004. doi: 10.1016/j.crmeth.2021.100004

Figure 1.

Figure 1

Sensory neuron isolation, plating, and automated stimulation

(A) DRG isolation and plating in 96-well format.

(B) Dissociated DRG neurons visualized by transmitted light (TL) (gray, left) and Fluo4 (green, middle) with overlay (right). Scale bars, 200 μm; inset shows detail.

(C) Cumulative distribution of cells per well for 13 plates individually (thin lines) and pooled (thick line).

(D) Average number of cells per well by plate (points), with mean ± SEM.

(E) Overview of liquid handling and imaging protocol for sequential stimuli.

(F) Traces from individual cells showing consecutive activation by either capsaicin (200 nM, left), menthol (300 μM, middle), or meATP (250 μM, right) and KCl (100 mM). Scale bars, 5 s, 0.5 dF/F.