Figure 6.

Development of high-throughput activation threshold analysis

(A) All-optical sensory neuron activation.

(B) Traces of light-dependent responses in individual sensory neurons. Scale bars (lines), 5 s, 0.5 dF/F.

(C) Percentage of cells responding to each optical train as mean ± SEM.

(D) Average response amplitude for positive (black) and negative (red) responses to each optical train as mean ± SEM.

(E) Percent change in light-sensitive sensory neurons after 7-day treatment by ChR2-targeting ASOs (red, green, blue, 10 μM) or scrambled ASO (gray, 10 μM) as mean ± SEM.

(F) Percentage of cells responding to each optical train after 7-day treatment by ChR2-targeting ASOs (red, green, blue) or scrambled ASO (gray) as mean ± SEM.

(G–L) Inhibition of optogenetic activation by tetrodotoxin (G) (TTX, 300 nM), ω-conotoxin MVIIA (H) (ωCtx, 3 μM), nifedipine (I) (Nif, 10 μM), retigabine (J) (Ret, 10 μM), linopirdine (K), (Lino, 50 μM), or gabapentin (L) (Gbp, 300 μM) with saline (dashed) as mean ± SEM.

(M) Percent change from saline in optogenetic activation by any stimulus train as mean ± SEM.