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. 2021 Jul 12;12:693066. doi: 10.3389/fmicb.2021.693066

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Copyright © 2021 Weitzel, Vegge, Pane, Goldman, Koshy, Porsby, Burguière and Schoeni.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow chart of the Lactobacillus spp. enumeration procedure. For each analysis, three plate counts are generated. The standard deviations for the plate count (S_p3) can be calculated from the triplicate plate count data to provide an estimate of the uncertainty from the plating and counting steps. The variance of the plate count can be subtracted from the variance covering the entire procedure to estimate the variance of the sample preparation (S_PREP). The values shown are for the example Lactobacillus spp.