TABLE 3.

The ANOVA experimental design and data for procedure qualification of the example, Lactobacillus spp.

Replicate	Counts in Log10 CFU/g
	Condition 1				Condition 2				Condition 3				Condition 4
	1	2	3	Average	1	2	3	Average	1	2	3	Average	1	2	3	Average
1	11.336	11.478	11.424	11.416	11.236	11.443	11.311	11.330	11.335	11.575	11.234	11.381	11.162	11.326	11.211	11.233
2	11.146	11.312	11.485	11.404	11.442	11.357	11.224	11.341	11.531	11.606	11.584	11.574	10.964	10.996	10.959	10.973
3	11.506	11.688	11.583	11.592	11.466	11.274	11.348	11.356	11.418	11.373	11.386	11.392	11.169	10.946	10.945	11.020
4	11.324	11.363	11.178	11.288	11.167	11.297	11.295	11.253	11.506	11.275	11.322	11.368	10.929	11.018	11.112	11.020
5	11.397	11.519	11.358	11.425	11.424	11.267	11.416	11.369	11.351	11.315	11.282	11.316	11.206	10.986	11.093	11.095
6	11.511	11.639	11.565	11.572	11.416	11.272	11.439	11.376	11.439	11.460	11.695	11.531	10.962	10.815	10.798	10.858
7	11.436	11.510	11.503	11.483	11.511	11.338	11.446	11.432	11.446	11.546	11.441	11.478	11.154	11.290	11.071	11.172
8	11.551	11.700	11.486	11.579	11.193	11.203	11.366	11.254	11.413	11.409	11.389	11.404	11.047	11.191	11.081	11.106
9	11.429	11.607	11.521	11.519	11.283	11.276	11.265	11.275	11.334	11.563	11.018	11.305	10.870	11.005	10.819	10.898
10	11.733	11.712	11.462	11.636	11.258	10.997	11.156	11.137	11.407	11.201	11.486	11.365	10.999	11.074	11.127	11.067
Std. Dev. (SC)				0.1080				0.0841				0.0888				0.1160
Variance (SC2)				0.0117				0.0071				0.0079				0.0134
Average (C)				11.491				11.312				11.411				11.044
Intermediate precision = Pooled Std. Dev. (SIP)				0.1001
Std. Dev. for single plate count (SP1)				0.1033
SEM for average of three plate counts (SP3)				0.05964
Std. Dev. for sample preparation (SPREP)				0.080393

The design includes four conditions with 10 replicates each and three plates per replicate. The standard deviation, variance, and average are calculated (equations are provided in Supplementary Appendix 2). The standard deviations for the four conditions are pooled to yield the intermediate precision. The standard deviation for an individual plate count is calculated. The SEM for the average of three plate counts is calculated, which allows the determination of the standard deviation for the sample preparation (see also Table 4 and Figure 4).