FIGURE 5.
(A) Sensitivity of strains to NHS. Viable bacterial counts were determined after 1 h of incubation at 37°C with rotation. Data are presented as percentage of survival, with 100% being the number of viable bacteria grown in heat-inactivated serum. All values are from triplicate samples and are representative of three independent experiments. (B) Kaplan–Meier survival curve showing the virulence of PBS, WT, and mutants in G. mellonella. The data show the percentage of survival (n = 30) of G. mellonella after inoculation with 106 CFU of bacteria. Survival curves were compared using the log-rank (Mantel–Cox) test. *P < 0.05; **P < 0.005; ***P < 0.001.