TABLE 2.
Primer name | Sequence | Product |
abaR(SphI)up-F | TATGCATGCTTACGCCACTGACTAAGAG | 1,190 bp |
abaR(BamHI)up-R | GCTGGATCCCGATAAGAGACCACTAACCT | |
abaR(BamHI)dw-F | TATGGATCCTTGAAGCGTAGGTCTAATCT | 1,136 bp |
abaR(PstI)dw-R | TATCTGCAGAAGGCGGTAACTGTAAGAA | |
abaI(PstI)dw-F | TATCTGCAGCGCAACTACAGCCATACT | 932 bp |
abaI(NotI)dw-R | TATGCGGCCGCGCCTCTTACCGACTTACG | |
abaI-F | AAAGTTACCGCTACAGGG | 435 bp |
abaI-R | CACGATGGGCACGAAA | |
abaR-F | TCCTCGGGTCCCAATA | 310 bp |
abaR-R | TAAATCTACCGCATCAA | |
abaI(EcoRI)-F | CCGGAATTCCGGGTGGAAGCACTTGTAATGAA | 654 bp |
abaI(XhoI)-R | CCGCTCGAGCGGCTCATCTTGCTCGGTCATA | |
abaR(EcoRI)-F | CCGGAATTCCGGCTACAAAAGCCCTAGCATT | 808 bp |
abaR(XhoI)-R | CCGCTCGAGCGGAAGATTAGACCTACGCTTCA | |
pME6032-F | CCTCATCAGTGCCAACATA | 843 bp |
pME6032-R | CATACTCTGCGACATCGTA |