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. Author manuscript; available in PMC: 2022 Jul 14.
Published in final edited form as: Chem Rev. 2021 May 26;121(13):7398–7467. doi: 10.1021/acs.chemrev.1c00009

Table 2.

Thermodynamic Parameters for 3WJs Formationsa

3WJs 1/Tm vs log (Ct)
ΔG°37 (kcal/mol) ΔH°37 (kcal/mol) ΔS°37 (eu) Tmb (°C)
2′-F RNA −36 ± 0.45 −200 ± 5.7 −520 ± 17 72.1
RNA −27 ± 0.58 −170 ± 13 −440 ± 39 60.4
DNA −15 ± 0.71 −220 ± 25 −650 ± 83 35.2
a

Parameters derived from 15% native TGGE. Reproduced with permission from ref 41. Copyright 2014 American Chemical Society.

b

Tm values for 3WJ strand concentrations of 10−6 M