FIG 7.
Depletion of tsA3Z2c-Z1b increased infectivity of progeny virus from tree shrew cells. (A) tsA3Z2c-Z1b genome sequences in TSLF-CD4 and tsA3Z2c-Z1b-KO mixed TSLF-CD4 cells were detected by PCR and then sequenced. Arrow indicates beginning of mutation. (B) tsA3Z2c-Z1b-knockout TSLF-CD4, TSLF-CD4, and TSLF cell lines were infected at an MOI of 2.0 with replication-competent HIV-1IIIB. Expression levels of p24 were detected by ELISA at 3 dpi. tsA3Z2c-Z1b and virus p24 protein from cells and supernatants were detected by Western blotting. (C) At 3 dpi, supernatant from panel B was normalized to 1 ng of p24 to infect C8166 cells. p24 expression was detected by ELISA at 3 dpi. (D) At 3 dpi, supernatant from panel B was normalized to 10 ng of p24 to infect TZM-bl cells. After 3 days, luciferase activity was detected. Positive-control virus HIV-1IIIB was made in C8166 cells.