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. 2021 Jul 26;95(16):e00852-21. doi: 10.1128/JVI.00852-21

FIG 5.

FIG 5

Flow cytometry gating strategy. (A) Mock: cells were infected with VACV-GFP1-9 and mock transfected. From left to right are the gated cell population, V5-stained cells from the gated cell population, and GFP fluorescence. (B) Negative interaction. Cells were infected with VACV-GFP1-9 and transfected with A16-HA-GFP10 and A28-V5-GFP11. From left to right: gated cell population, V5-stained cells from gated cell population, GFP expression in V5-stained population. (C) Positive interaction. Cells were infected with VACV-GFP1-9 and transfected with H2-HA-GFP10 and A28-V5-GFP11. The images as described for panel B.