Table 1. Summary of Pch2 subcellular localization, Hop1 chromosomal distribution and checkpoint activity in different situations.
| Pch2 subcellular localization | Hop1 localization | ||||||
|---|---|---|---|---|---|---|---|
| Relevant Genotype(1) | Chromosomes | Nucleolus (rDNA) | Cytoplasm | Other | Chromosome axes | Nucleolus (rDNA) | Checkpoint Activity |
| wild type | + (faint foci) | + | + | - | foci | - | na |
| zip1Δ | - | + | + | - | linear | - | + |
| zip1Δ pch2Δ | - | - | - | - | discontinuous | + | - |
| zip1Δ orc1-3mAID | - | - | + | - | linear(2) | +(2) | + |
| zip1Δ NES-PCH2 | - | -/+ | + | - | linear | + | + |
| zip1Δ NLS-PCH2 (hem) | - | + | -/+ | nucleoplasm | discontinuous | - | -/+ |
| zip1Δ NLS-PCH2 (hom) | - | + | -/+ | nucleoplasm | discontinuous | - | - |
| zip1Δ dot1Δ | + (widespread) | + | + | - | discontinuous(3) | +/-(3) | - |
| zip1Δ orc1-3mAID dot1Δ | - | - | + | - | nd | nd | + |
| zip1Δ nup2Δ | - | +/- | + | - | nd | nd | + |
| zip1Δ PIL1-GBP | - | - | - | eisosomes | strong linear | + | + |