Fig 2. PBT434 toxicity in brain microvascular endothelial cells.

hBMVEC were incubated with increasing concentrations of PBT434 for 24h. Cell viability was then assessed by standard MTT assay that interrogates physiologic mitochondrial function, using Triton-X as a positive control for cell killing. Data are represented as mean ± SEM, n = 3 biological replicates. Statistical significance was determined using Welch’s ANOVA with Dunnett’s T3 multiple comparison test compared to control.