Fig 9. ⁵⁵Fe²⁺ uptake by hBMVEC is inhibited by complexing with PBT434, but not with PBT434 pre-treatment.

hBMVEC were loaded with 1 μM ⁵⁵Fe²⁺ at 37°C for 3h in cells in complex with 20 μM PBT434 or PBT434-met in the loading media (A-C) or pre-treated with PBT434 or PBT434-met for 24h prior to the start of uptake (B-D). Cells were lysed over the indicated period of time and the amount of ⁵⁵Fe²⁺ accumulated at each timepoint (A, B) and after 3h (C, D) was calculated. Data are represented as mean ± SEM, n = 4 experimental replicates/time point. The rates of ⁵⁵Fe²⁺ accumulation between 0.5-3h were determined using linear regression analysis. Statistical significance was determined using one-way ANOVA and Tukey’s multiple comparison test. *, statistically significantly different from control or as indicated; *, p < 0.05; **, p < 0.01; ***, p < 0.001.