Fig 11. PBT434 stimulates ferroportin-dependent ⁵⁵Fe²⁺ efflux.

hBMVEC were loaded with 1 μM ⁵⁵Fe²⁺ at 37°C for 24h in the absence (-PR73) or presence of mini-hepcidin PR73 (+PR73, 1.4 μM). Iron efflux was then initiated in efflux medium alone (control, closed circles), or media containing PBT434 (20 μM, open squares). The amount of ⁵⁵Fe lost from the cells after 6h incubation was quantified; the data are presented as the percent loss of the initial cell-associated ⁵⁵Fe²⁺. Data are represented as mean ± SEM, n = 6 biological replicates/condition. Statistical significance was determined using two-way ANOVA and Tukey’s multiple comparison test. **, p < 0.01; ****, p < 0.0001 compared to control or as indicated.