Pair	Gene	Capture/ Detection	Start (cDNA)	Stop (cDNA)	length	seq	Tm
1	N	capture	955	995	40	BiotinTGTCAAGCAGCAGCAAAGCAAGAGCAGCATCACCGCCATTG	70.5
		detection	1010	1044	34	Cy5AGGAGAAGTTCCCCTACTGCTGCCTGGAGTTGAAT	66.8
		Positive control			90	ATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACA
2	ORF1ab	capture	10740	10770	30	BiotinGTCAGAATGTGTGGCATAAGAATAGAATAAT	56.5
		detection	10855	10890	35	Cy5GCTTTAGGGTTACCAATG TCGTGAAGAACTGGGAAT	64.4
		Positive control			139	ATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGAATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATTCTGAC
3	ORF1a	capture	21551	21587	36	BiotinTACAACTATCGCCAGTAA CTTCTATGTCAGATTGATGTGA	68.9
		detection	21628	21667	39	Cy5CGCACTACAGTCAATACAAGCACCAAGGTCACGGGGT	63.5
		Positive control			117	TCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCG
4	ORF1a	capture	26634	26674	40	BiotinATAGTAGTTGTCTGATTGTCCTCACTGCCGTCTTGTTGACC	67.5
		detection	26682	26722	40	Cy5TGTTGACTATCATCATCTAACCAATCTTCTTCTTGCTCTTC	63.5
		Positive control			89	GAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAGACGGCAGTGAGGACAATCAGACAACTACTAT
5	ORF3a	capture	4166	4206	40	BiotinGCAAGAAGTAGACTAAAGCATAAAGATAGAGAAAAGGGGCT	64.5
		detection	4215	4249	34	AF647AGCAGCAACGAGCAAAAGGTGTGAGTAAACTGT TA	63.3
		Positive control			84	TAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTATGCTTTAGTCTACTTCTTGC
6	ORF1ab/ helicase	capture	13310	13335	25	BiotinAATCACCAGCATTTGTCCAGTCACAT	56.4
		detection	13354	13380	26	AF647TCAGTAACATTATCGCTACCAACACAT	55.2
		Positive control			71	ATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATT