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. 2021 Jul 14;19(7):e3001326. doi: 10.1371/journal.pbio.3001326

Fig 4. Expression of NbPT5b and NbBCP1b genes is induced under colonisation with Rhizophagus irregularis in Nicotiana benthamiana.

Fig 4

(a) qRT-PCR analysis of NbPT5b and NbBCP1b shows that gene expression is induced 2 wpi with R. irregularis and increases with time and degree of colonisation. Y axes indicate expression levels relative to N. benthamiana’s elongation factor 1 alpha (NbEF). Expression of RiBTub is used as a fungal biomass marker for root colonisation. Raw qRT-PCR values can be found in S3 Data. (b and c) GUS staining of NbPT5b-GUS and NbBCP1b-GUS expressing N. benthamiana roots 4 weeks after inoculation with R. irregularis. GUS activity can only be observed in root areas colonised by R. irregularis and is more predominant in arbuscule-containing cells (white arrows). Scale bar, 500 μm. qRT-PCR, quantitative real-time polymerase chain reaction; RiBTub, R. irregularis β-tubulin; wpi, weeks postinoculation.