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. 2021 Jul 15;207(2):534–541. doi: 10.4049/jimmunol.2000072

FIGURE 1.

FIGURE 1.

Binding of FH and rFHR-5 to surface-immobilized GAGs. (A) FH and (B) rFHR-5 showed dose-dependent binding correlating with the degree of GAG sulfation and lack of binding to the nonsulfated GAG HA. (C) FH and (D) rFHR-5 binding to surface-immobilized, selectively desulfated GAGs showing reduction of binding after desulfation. The data are representative of more than 10 independently performed experiments using the GAGs and representative of two independently performed experiments using the selectively desulfated GAGs. Data points represent mean of two replicates with SD. Hep 2-O-deS, heparin desulfated at the 2-O position of the iduronic acid; Hep 6-O-deS, heparin desulfated at the 6-O position of the iduronic acid; Hep N-deS, heparin desulfated at the N-position of the glucosamine followed by N-reacetylation.