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Protein concentration-dependent binding of rFHR-5 to HS and heparin oligosaccharide microarrays. FHR-5 was overlaid at 10, 5, 1, and 0.2 µg of protein per milliliter onto microarrays that contain HS and heparin NGL probes with selected saccharide chain lengths as indicated. Binding signals (fluorescence intensities) represent mean of quadruplicate spots at 5 fmol/spot and error bars represent SD. Binding data shown are representative of at least two independent experiments.
