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. 2021 Jul 15;207(2):483–492. doi: 10.4049/jimmunol.2000565

FIGURE 1.

FIGURE 1.

Transfection with miR-1264 mimic and miR-107 mimic increases miR-1264 and miR-107 levels, respectively, in hAM. hAM were collected from control subjects (Con; n = 17) and from subjects with an AUD (Alc; n = 17). (A and B) miRNA levels of Nox1-related miR-1264 and miR-1982 (A) or Nox2-related miR-103 and miR-107 (B) were measured in hAM by quantitative RT-PCR (qRT-PCR; in duplicate), normalized to 5U ribosomal mRNA, and expressed as mean ± SEM, relative to control. (C and D) hAM were transiently transfected with 50 nM miR-1264 mimic (miR-1264-M), miR-107 mimic (miR-107-M), or scrambled miR mimics (miR-Scr) for 24 h. miR-1264 (C) or miR-107 (D) levels were measured in hAM (n = 17 per group) by qRT-PCR (in duplicate), normalized to 5U ribosomal mRNA, and expressed as mean ± SEM, relative to control. (E and F) MH-S cells were transiently cotransfected with either scrambled (Scr) or miR-1264 or miR-107 mimics along with Nox1 (E) or Nox2 (F) 3' UTR luciferase reporter constructs for 24 h. MH-S cells were then treated with either Con or EtOH (0.08%) conditions for 3 d (n = 6 per group). MH-S luciferase activity was measured in duplicate, normalized to protein concentrations, and expressed as mean ± SEM, relative to control (n = 6 per group). *p < 0.05 versus Con or Con Scr, Φp < 0.05 versus Alc Scr or EtOH Scr.