Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila melanogaster) | gnu | FLYB: FBgn0001120 | ||
| Gene (D. melanogaster) | png | FLYB: FBgn0000826 | ||
| Gene (D. melanogaster) | plu | FLYB: FBgn0003114 | ||
| Gene (D. melanogaster) | Bic-C | FLYB: FBgn0000182 | ||
| Gene (D. melanogaster) | tral | FLYB: FBgn0041775 | ||
| Gene (D. melanogaster) | me31b | FLYB: FBgn0004419 | ||
| Strain, strain background (D. melanogaster) | WT: Oregon R | N/A | ||
| Genetic reagent (D. melanogaster) | gnu-wt-gfp[1-5] | Hara et al., 2017 | Genotype: w;P{w[+mC], gnu-wt-gfp}; gnu[305]/
TM3, Sb |
|
| Genetic reagent (D. melanogaster) | gnu-wt-gfp[1-4] | Hara et al., 2017 | Genotype: w, P{w[+mC], gnu-wt-gfp};;gnu[305]/TM3, Sb | |
| Genetic reagent (D. melanogaster) | gnu-wt-gfp[1-8] | Hara et al., 2017 | Genotype: w;P{w[+mC], gnu-wt-gfp}; gnu[305]/
TM3, Sb |
|
| Genetic reagent (D. melanogaster) | gnu-9A-gfp[2-7] | Hara et al., 2017 | Genotype: w;P{w[+mC], gnu-9A-gfp}; gnu[305]/
TM3, Sb |
|
| Genetic reagent (D. melanogaster) | gnu-9A-gfp[2-8] | Hara et al., 2017 | Genotype: w;P{w[+mC], gnu-9A-gfp}; gnu[305]/
TM3, Sb |
|
| Genetic reagent (D. melanogaster) | tral-gfp[89] | The Flytrap Project; Morin et al., 2001; PMID:11742088 | Flytrap:G00089; DGRC:110584; RRID:DGGR_110658 | Genotype: w[*];P{w[+mC]=PTT-un1}
G00089 |
| Genetic reagent (D. melanogaster) | me31b-gfp | Nakamura et al., 2001 | Genotype: w, P{[W[+mC]], me31b-gfp} | |
| Genetic reagent (D. melanogaster) | Bic-C-gfp[v318872] | Sarov et al., 2016 | RRID:VDRC_v318872 | Transgene carries dsRed marker. Genotype: w;;PBac{fTRG01264.sfGFP-TVPTBF}VK00033 |
| Genetic reagent (D. melanogaster) | Bic-C-gfp[v318334] | Sarov et al., 2016 | RRID:VDRC_v318334 | Transgene carries dsRed marker. Genotype: w;;PBac{fTRG01264.sfGFP-TVPTBF}VK00033 |
| Genetic reagent (D. melanogaster) | gnu-ΔSAM-gfp[3-2] | This paper | Genotype: w;P{w[+mC], gnu-ΔSAM-gfp}; gnu[305]/
TM3, Sb |
|
| Genetic reagent (D. melanogaster) | gnu-ΔSAM-gfp[3-8] | This paper | Genotype: w;P{w[+mC], gnu-ΔSAM-gfp}; gnu[305]/
TM3, Sb |
|
| Genetic reagent (D. melanogaster) | gnu-mKATE2 | This paper | Genotype: w;P{w[+mC], gnu-mKATE2}; gnu[305]/
TM3, Sb |
|
| Genetic reagent (D. melanogaster) | BiC-C[4] | Schüpbach and Wieschaus, 1991 | RRID:BDSC_3248 | Genotype: Bic-C[4], cn[1], exu[1], bw[1]/CyO |
| Genetic reagent (D. melanogaster) | Bic-C[PE37] | Schüpbach and Wieschaus, 1991 | Genotype: Bic-C[PE37], bw[1]/CyO | |
| Genetic reagent (D. melanogaster) | gnu[305] | Freeman and Glover, 1987 | FLYB: FBal0005121 | Genotype: ru, gnu[305], th, st, roe, p(p), e(s), ca/TM3, Sb |
| Genetic reagent (D. melanogaster) | png[1058] | Shamanski and Orr-Weaver, 1991; PMID:1913810 | RRID:BDSC_38437 | Genotype: y[1], png[1058], w[*]/FM6 |
| Genetic reagent (D. melanogaster) | h2av-gfp | RRID:BDSC_24163 | Genotype: w[*]; P{w[+mC]=His2Av-EGFP.C}2/
SM6a |
|
| Genetic reagent (D. melanogaster) | pdi-gfp | RRID:BDSC_6839 | Genotype: w[1118]; P{w[+mC]=PTT-GA}Pdi[G00198]/TM3, Sb[1] Ser[1] | |
| Genetic reagent (D. melanogaster) | me31b-gfp; Bic-C[305]/Xa | This paper | Generated from cross between me31b-gfp females and Bic-C[4] males. Genotype: w, P{[W[+mC]], me31b-gfp}; Bic-C[4]/Xa | |
| Genetic reagent (D. melanogaster) | gnu-gfp/+; Bic-C[305]/Sco | This paper | Generated from cross between gnu-wt-gfp[1-4] females and Bic-C[4] males. Genotype: w;P{w[+mC], gnu-wt-gfp}/+; Bic-C[4]/Sco | |
| Genetic reagent (D. melanogaster) | me31b-gfp;; gnu[305]/TM3 | This paper | Genotype: w, P{[W[+mC]], me31b-gfp};; gnu[305]/TM3 | |
| Genetic reagent (D. melanogaster) |
png[1058]/
FM0; gnu-wt-gfp; gnu[305]/TM3 |
This paper | Genotype: png[1058]/FM0; P{w[+mC], gnu-wt-gfp}/+; gnu[305]/TM3 | |
| Genetic reagent (D. melanogaster) | gnu-gfp/+; BicC[4]/Bic-C[PE37] | This paper | Generated from genetic cross between gnu-gfp/+; BicC[4]/Sco males and Bic-C[PE37] females. |
|
| Genetic reagent (D. melanogaster) | plu-gfp[5-04] | This paper | PLU-GFP transgene; good expression; rescues homozygous plu. Genotype: y,w; P{plu C/
GFP E.1} |
|
| Genetic reagent (D. melanogaster) | png-gfp[F-041] | This paper | PNG-GFP transgene; good expression; rescues homozygous png[1058]. Genotype: y,w; P{png-gfp} | |
| Genetic reagent (D. melanogaster) | twine/CyO | Other | RRID:BDSC_4274; RRID:Kyoto_107663 | Sterile males due to spermlessness. Genotype: twe[1] cn[1] bw[1]/CyO |
| Antibody | Guinea pig polyclonal anti-GNU | Lee et al., 2003 | PMID:14665672 | (1/5000) in TBS-T |
| Antibody | Rabbit polyclonal anti-BIC-C | P. Lasko (McGill University) |
(1/2000) in TBS-T | |
| Antibody | Guinea pig polyclonal anti-GFP | M. Pardue (MIT) |
(1/5000) in TBS-T | |
| Antibody | Rat monoclonal anti-αTUB YOL1/34 | AbD Serotec (Bio-Rad) |
(1/1000) in TBS-T | |
| Antibody | Mouse monoclonal anti-CycB | Developmental Studies Hybridoma Bank | DSHB Cat#F2F4 RRID:AB_528189 |
(1/100) in Hikari Solution B |
| Antibody | Rat monoclonal anti-MBP | Sigma-Aldrich | Sigma-Aldrich: SAB4200082 |
(1/2000) in Hikari Solution |
| Antibody | Rabbit polyclonal anti-GST labeled with HRP | MBL | MBL:PM013-7; RRID:AB_10598029 | (1/5000) in Hikari solution |
| Antibody | Rabbit polyclonal anti-ME31B | Nakamura et al., 2001 | PMID:27791980; RRID:AB_2568986 | (1/10,000) in TBS-T |
| Antibody | Donkey polyclonal HRP-conjugated anti-guinea pig IgG |
Jackson ImmunoResearch | Jackson Immuno Research:706-035-148: RRID:AB_2340447 | |
| Antibody | Goat polyclonal HRP-conjugated anti-mouse IgG |
Jackson ImmunoResearch | Jackson Immuno Research:115-035-164: RRID:AB_2338510 | |
| Antibody | Goat polyclonal HRP-conjugated anti-rat IgG | Jackson ImmunoResearch | Jackson Immuno Research:112-035-062: RRID:AB_2338133 | |
| Antibody | Donkey polyclonal HRP-conjugated anti-rabbit IgG |
Jackson ImmunoResearch | Jackson Immuno Research: 711-035-152; RRID:AB_10015282 |
|
| Antibody | Recombinant Nanobody gba488-100 (GFP-Booster Atto488) |
Chromotek | Booster for GFP fluorescence (1/400) in diluent buffer | |
| Recombinant DNA reagent | pCasPeR4_gnu-wt-gfp | Hara et al., 2017 | RRID:Addgene_113005 | |
| Recombinant DNA reagent | pCasPeR4_gnu-ΔSAM-gfp | This paper | Generated from pCasPeR4_gnu-wt-gfp | |
| Recombinant DNA reagent | pCasPeR4_gnu-mkate2 | This paper | Generated from pCas
PeR4_gnu-wt-gfp |
|
| Commercial assay or kit | gtm_20 anti-GFP magnetic beads | Chromotek | GFP immunoprecipitation | |
| Chemical compound, drug | HIKARI signal enhancer | Nacalai | Nacalai: 02270–81 |
Signal Enhancer HIKARI for Western Blotting and ELISA |
| Software, algorithm | Imaris (Bitplane) | Bitplane | Imaging data visualization and analysis | |
| Software, algorithm | FIJI (ImageJ) | ImageJ | Imaging data visualization and analysis | |
| Software, algorithm | Scaffold (version 4) | Proteome Software | Proteomic data visualization and analysis |