Table 1. Interactors with GNU in mature oocytes identified through IP-MS.
Identified protein | Total spectrum count | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No GFP | GNUWT-GFP | GNU9A-GFP | GNUΔSAM-GFP | H2Av-GFP | GNUWT-GFP (+RNase A) |
||||||||
Rep 1 | Rep 2 | Rep 1 | Rep 2 | Rep 3 | Rep 1 | Rep 2 | Rep 1 | Rep 2 | Rep 1 | Rep 2 | Rep 3 | ||
GFP | – | – | 319 | 376 | 307 | 62 | 52 | 353 | 520 | 358 | 49 | 31 | 467 |
GNU | – | – | 179 | 178 | 220 | 81 | 90 | 152 | 109 | 3 | – | – | 205 |
BIC-C | – | – | 92 | 13 | 73 | 5 | – | – | – | – | – | – | 90 |
TYF | – | – | 64 | 6 | 11 | 9 | – | 45 | 5 | 11 | 3 | – | 38 |
YPS | – | – | 40 | 11 | 23 | 10 | – | 38 | 2 | 28 | – | – | – |
TWS | – | – | 39 | 9 | 22 | 10 | 3 | 92 | 14 | – | – | – | 32 |
TRAL | 3 | 5 | 37 | 7 | 17 | 5 | 5 | 22 | 2 | 20 | 2 | – | 11 |
PP2A-29B | – | – | 27 | 4 | 30 | 8 | 4 | 52 | 7 | 5 | – | – | 24 |
CUP | – | – | 20 | 5 | 7 | 7 | 4 | 9 | – | 4 | – | – | 9 |
ATX-2 | – | – | 20 | 6 | 9 | – | – | 23 | 6 | 6 | – | – | 16 |
ME31B | 2 | 2 | 11 | 3 | 6 | 6 | 8 | 6 | 2 | 9 | – | – | 6 |
MTS | – | – | 10 | – | 10 | 3 | – | 42 | 5 | – | – | – | 8 |
RPL36A | – | – | 7 | 5 | 5 | – | – | 3 | 2 | – | – | – | 7 |