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. 2021 Jul 12;10:e68268. doi: 10.7554/eLife.68268

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Genetic reagent (Drosophila melanogaster) Canton-S Shanghai Institute of Biochemistry and Cell Biology Cat#BCF47  
Genetic reagent (Drosophila melanogaster) w1118 Bloomington Drosophila Stock Center Cat#5905RRID:BDSC_5905  
Genetic reagent (Drosophila melanogaster) elav-Gal4 Bloomington Drosophila Stock Center Cat#8765RRID:BDSC_8765  
Genetic reagent (Drosophila melanogaster) octβ2R-Df Bloomington Drosophila Stock Center Cat#56254RRID:BDSC_56254  
Genetic reagent (Drosophila melanogaster) octβ3RMB04794 Bloomington Drosophila Stock Center Cat#24819RRID:BDSC_24819  
Genetic reagent (Drosophila melanogaster) octβ1R Koon and Budnik, 2012    
Genetic reagent (Drosophila melanogaster) octβ2Rf05679 Lim et al., 2014 Cat#18896RRID:BDSC_18896  
Genetic reagent (Drosophila melanogaster) oambdel Deng et al., 2019    
Genetic reagent (Drosophila melanogaster) oamb-Gal4 Zhou et al., 2012    
Genetic reagent (Drosophila melanogaster) octα2Rattp Deng et al., 2019    
Genetic reagent (Drosophila melanogaster) octα2R-Gal4 Deng et al., 2019    
Genetic reagent (Drosophila melanogaster) octβ2R-Gal4 Deng et al., 2019    
Genetic reagent (Drosophila melanogaster) UAS-trpA1 Hamada et al., 2008    
Genetic reagent (Drosophila melanogaster) UAS-Shibirets Kitamoto, 2001    
Genetic reagent (Drosophila melanogaster) oct-tyrRGal4 This paper   Mutant allele; Materials and methods, ‘Fly strains’
Genetic reagent (Drosophila melanogaster) UAS-VdOctβ2R This paper   Mutant allele; Materials and methods, ‘Fly strains’
Genetic reagent (Drosophila melanogaster) UAS-AmOctβ2R This paper   Mutant allele; Materials and methods, ‘Fly strains’
Genetic reagent (Drosophila melanogaster) UAS-AmOctβ2RV3X This paper   Mutant allele; Materials and methods, ‘Fly strains’
Chemical compound, drug Amitraz Sigma Cat#45323  
Chemical compound, drug N2-(2,4-Dimethylphenyl)-N1-methyformamidine Sigma Cat#BP641  
Chemical compound, drug 2,4-Dimethylaniline Sigma Cat#301493  
Chemical compound, drug (±)-Octopamine hydrochloride Sigma Cat#68631  
Chemical compound, drug 2,4-Dimethylformanilide AccuStandard Cat#P-1100S-CN  
Chemical compound, drug Piperonyl butoxide Aladdin Cat#P113864  
Chemical compound, drug S,S,S-Tributylphosphorotrithioate Aladdin Cat#T114221  
Chemical compound, drug Diethyl maleate Aladdin Cat#D104017  
Chemical compound, drug Poly-D-lysine Sigma Cat#P0296  
Chemical compound, drug Sucrose Sinopharm Cat#10021418  
Chemical compound, drug Agarose Sinopharm Cat#63005518  
Cell lines HEK 293 The Cell Bank of Type Culture Collection of Chinese Academy of Sciences Cat#GNHu43 https://www.cellbank.org.cn/
Recombinant DNA reagent Plasmid: pcDNA3.1-VdOAMB This paper   See ‘Construction of expression plasmids’
Recombinant DNA reagent Plasmid: pcDNA3.1-VdOctβ2R This paper   See ‘Construction of expression plasmids’
Recombinant DNA reagent Plasmid: pcDNA3.1-VdOctβ2RI40F This paper See ‘Construction of expression plasmids’
Recombinant DNA reagent Plasmid: pcDNA3.1-VdOctα2R This paper   See ‘Construction of expression plasmids’
Recombinant DNA reagent Plasmid: pcDNA3.1-VdOct-tyrR This paper   See ‘Construction of expression plasmids’
Recombinant DNA reagent Plasmid: pcDNA3.1-AmOAMB This paper   See ‘Construction of expression plasmids’
Recombinant DNA reagent Plasmid: pcDNA3.1-AmOctβ2R This paper   See ‘Construction of expression plasmids’
Recombinant DNA reagent Plasmid: pcDNA3.1-AmOctβ2RV3X This paper   See ‘Construction of expression plasmids’
Recombinant DNA reagent Plasmid: pcDNA3.1-AmOctα2R This paper   See ‘Construction of expression plasmids’
Recombinant DNA reagent Plasmid: pcDNA3.1-AmOct-tyrR This paper   See ‘Construction of expression plasmids’
Recombinant DNA reagent Plasmid: pcDNA3.1-DmOAMB This paper   See ‘Construction of expression plasmids’
Recombinant DNA reagent Plasmid: pcDNA3.1-DmOctβ2R This paper   See ‘Construction of expression plasmids’
Recombinant DNA reagent Plasmid: pcDNA3.1-DmOctα2R This paper   See ‘Construction of expression plasmids’
Recombinant DNA reagent Plasmid: pcDNA3.1-DmOct-tyrR This paper   See ‘Construction of expression plasmids’
Recombinant DNA reagent Plasmid: pcDNA3.1-RmOctβ2R This paper   See ‘Construction of expression plasmids’
Recombinant DNA reagent Plasmid: pcDNA3.1-RmOctβ2RI61F This paper   See ‘Construction of expression plasmids’
Software SoftMax Pro software (v. 7.1.2.0) Molecular Devices   https://www.moleculardevices.com/
Software Molecular Operating Environments (MOE, 2015.10) Chemical Computing Group   https://www.chemcomp.com/
Software Prism 7.0 GraphPad GraphPad Prism, RRID:SCR_002798  
Other DMEM media ThermoFisher Scientific Cat#10566016  
Other Lipofectamine 2000 ThermoFisher Scientific Cat#11668019  
Other 96 well polystyrene microplates ThermoFisher Scientific Cat#165305  
Other 0.25% Trypsin-EDTA ThermoFisher Scientific Cat#25200072  
Other Fura 2-AM and Pluronic F-127 Dojindo Molecular Technologies Cat#F025