Fig. 2.

Time course of ultrasound‐mediated gene transfer into a biofilm. Green fluorescence signal (ex. 485nm, em. 510nm) and bright‐field imaging of biofilm samples after 120 h with (A) the addition of both plasmid and ultrasound treatment (+P/+U), (B) only the addition of plasmid (+P/−U), (C) only ultrasound treatment (−P/+U) and (D) no plasmid and no ultrasound treatment (−P/−U). Green fluorescence signal and bright‐field imaging for biofilm samples with both addition of plasmid and ultrasound treatment after (E) 5 h, (F) 24 h, (G) 48 h and (H) 120 h.