Fig. 7.

Mining of a cryptic BGC in B. plantarii DSM9509.

A. HPLC‐MS analysis (BPC) of extracts from (a) DSM 9509 wild type, (b) BGC4 inactivated mutant of DSM9509‐DSM9509::P_Apra‐BGC4Δmodule2 and (c) BGC4 activated mutant of DSM 9509‐DSM9509::P_Apra‐BGC4.

B. Structure of BGC4 and NRPS module architecture of hptC. The structure shows the promoter (P‐Apra) inserted upstream of the first gene in the BGC. The predicted NRPS modules and A domain are shown for hpt. A, adenylation domain; C, condensation domain; C/E, dual condensation/epimerization domain; C_S, starter condensation domain; T, peptidyl carrier domain; TE, thioesterase domain.

C. Complete structures of compounds 1–7 produced by DSM9509::P_Apra‐BGC4.