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. 2021 Jan 18;14(4):1433–1444. doi: 10.1111/1751-7915.13748

Fig. 1.

Fig. 1

Construction of different expression vectors and confirmation of different transgenic strains.

A. Construction of different vectors used in this study. a, Expression vector for pr1A. b, Expression vector for BjαIT. c, Expression vector for the double‐strand RNA (dsRNA) targeting host‐specific‐gnbp3. d, Expression vector expressing both pr1A and BjαIT. E, Expression vectors expressing pr1A, BjαIT and dsRNA targeting host‐specific gnbp3.

B. PCR and reverse transcription PCR (RT‐PCR) analysis of different transgenic strains with primers for bar. 1, Mr‐pr1A; 2, Mr‐BjαIT; 3, Mr‐pr1A‐BjαIT; 4, Mr‐GMgnbp3; 5, Mr‐LMgnbp3; 6, Mr‐PXgnbp3; 7, Mr‐TMgnbp3; 8, Mr‐pr1A‐BjαIT‐GMgnbp3; 9, Mr‐pr1A‐BjαIT‐LMgnbp3; 10, Mr‐pr1A‐BjαIT‐PXgnbp3; 11, Mr‐pr1A‐BjαIT‐TMgnbp3; 12, Mr‐WT.