Fig. 3.

Survival trends and LT₅₀ of the different transgenic strains in four insect species.

A. Time‐mortality trends of four insect species infected with the different transgenic strains. Third‐instar Plutella xylostella, fourth‐instar Tenebrio molitor, and fifth‐instar Galleria mellonella and Locusta migratoria larvae were used to assess the virulence of the fungal transgenic strains. Conidia were applied by immersing larvae into an aqueous 10⁵ conidia ml^‐1 suspension. Each treatment consisted of three replicates (60 larvae per replicate).

B. LT₅₀ values of the transgenic strains in four insect species. Different lowercase letters marked on the bars in each graph denote significant differences (P < 0.05). Inset in each graph are images of insect cadavers maintained at 28°C and high humidity for 12 days. Error bars, standard deviation from six replicate assays.