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. 2021 Jan 18;14(4):1433–1444. doi: 10.1111/1751-7915.13748

Fig. 4.

Fig. 4

Pr1A enzyme activity and Western blot analyses of protein expression in the different transgenics.

A. Pr1A enzyme activity of the different transgenic strains grown in Sabouraud dextrose broth (SDB) or cuticle‐inductive medium. Error bars, standard deviation from six replicate assays.

B. Western blot analysis of Pr1A, BjαIT and GNBP3 expression. Pr1A expression in the different transgenic strains (1, Mr‐WT; 2, Mr‐pr1A; 3, Mr‐pr1A‐BjαIT; 4, Mr‐pr1A‐BjaIT‐GMgnbp3; 5, Mr‐pr1A‐BjaIT‐LMgnbp3; 6, Mr‐pr1A‐BjaIT‐PXgnbp3; 7, Mr‐pr1A‐BjaIT‐TMgnbp3) grown in SDB or cuticle‐inductive medium; BjαIT expression in different transgenic strains (1, Mr‐WT; 2, Mr‐BjaIT; 3, Mr‐pr1A‐BjaIT; 4, Mr‐pr1A‐BjaIT‐GMgnbp3; 5, Mr‐pr1A‐BjaIT‐LMgnbp3; 6, Mr‐pr1A‐BjaIT‐PXgnbp3; 7, Mr‐pr1A‐BjaIT‐TMgnbp3) grown in SDB or cuticle‐inductive medium; GNBP3 expression in the different insect species infected with different transgenic strains (1, Water; 2, Mr‐WT; 3, Mr‐x, x = PXgnbp3, TMgnbp3 or GMgnbp3; 4, Mr‐pr1A‐BjaIT‐x, x = PXgnbp3, TMgnbp3 or GMgnbp3) at 72 h.