Fig. 2.

Effects of the minicell‐encapsulated dsRNAs (ME‐dsRNAs) on mycelial growth and silencing of respective RNAi‐machinery related‐ genes in Botryotinia fuckeliana at different time points.

A. Inhibition of fungal growth in response to ME‐dsRNAs targeting RNAi‐machinery related‐ genes dicer‐like protein 1 (DCL1), and dicer‐like protein 2 (DCL2) of B. fuckeliana at different concentrations.

B. Mycelial growth inhibition rate in response to ME‐DCL1 and ME‐DCL2.

C and D. Relative normalized expressions of the B. fuckeliana genes in responses to ME‐DCL1 and ME‐DCL2 (E); DCL1, (F); DCL2. Data represents the mean ± SEM. Bars labelled with different letters are significantly different at P < 0.05 according to Duncan’s multiple range test. Asterisks indicate significant differences between minicells and ME‐dsRNA‐treated sample at each time point according to t‐test; *P < 0.05, **P < 0.01, ***P < 0.001.