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. Author manuscript; available in PMC: 2021 Jul 26.
Published in final edited form as: Cell Rep. 2021 Jul 6;36(1):109338. doi: 10.1016/j.celrep.2021.109338

Figure 6. Ser-1459 mutation regulates the gating of GluN1/2A diheteromeric NMDARs.

Figure 6.

(A) Continuous sweep of single-receptor currents mediated by GluN1/2AWT (top, blue), GluN1/2AS1459G (middle, red), and GluN1/2AS1459D (bottom, green) receptors recorded in the presence of 1 mM glutamate, 0 mM extracellular Mg2+, and 100 μM glycine at −70 mV. Note the similar current amplitudes but longer active durations in the mutant receptors.

(B and C) Summary box-and-whisker plots of intra-activation PO (B) and active duration (C) of individual receptors. Boxes indicate the median, 25th, and 75th percentiles, and the whiskers mark the minimum and maximum values (WT, n = 119 single receptor activations from 8 membrane patches; S1459G, n = 101 activations from 5 patches; S1459D, n = 149 activations from 16 patches). *p < 0.05, ***p < 0.001 using Kruskal-Wallis one-way ANOVA with a Dunn’s multiple comparison test.