KEY RESOURCES TABLES
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Alexa 488-conjugated goat anti-chicken IgY antibody | Thermo Scientific | Cat# A32931TRRRID: AB_2866499 |
| Alexa 568-conjugated goat anti-rabbit IgG antibody | Thermo Scientific | Cat# A-11036RRID: AB_10563566 |
| Alexa 647-conjugated goat anti-rabbit IgG antibody | Thermo Scientific | Cat# A-21245RRID: AB_2535813 |
| β-actin (mouse monoclonal, clone C4, used 1:5000 in WB) | Santa Cruz Biotechnology | Cat# sc-47778; RRID:AB_2714189 |
| CaMKII (pan) (rabbit monoclonal, clone D11A10, used 1:1000 in WB) | Cell Signaling Technology | Cat# 4436RRID:AB_10545451 |
| Fab fragment donkey anti-rabbit IgG | Jackson ImmunoResearch | Cat# 711-007-003RRID: AB_2340587 |
| GFP (rabbit polyclonal, used 1:5000 in WB) | Proteintech | Cat# 50430-2-APRRID:AB_11042881 |
| GFP (rabbit polyclonal, JH4030, used 1:250 in ICC) | A gift from R. Huganir (Johns Hopkins University) (Anggono et al., 2011) | N/A |
| GFP (chicken polyclonal, used 1:5000 in WB & ICC) | Aves labs | Cat# GFP-1020RRID:AB_10000240 |
| GFP (mouse monoclonal, used 1:1000 in ICC) | Abcam | Cat# ab1218RRID: AB_298911 |
| GluN2A (rabbit monoclonal, clone A12W, used 1:3000 in WB, 1:200 in ICC) | Millipore | Cat# 04-901RRID:AB_1163481 |
| GluN2A phospho-S1459 (1:1000 used in WB, 2ul used in co-IP) | Mota Vieira et al., 2020 | N/A |
| GST (mouse monoclonal, clone 3G12B10, used 1:5,000 in WB) | Proteintech | Cat# 66001-2-lg |
| MAP2 (chicken polyclonal, used 1:2000 in ICC) | Abcam | Cat# ab92434RRID:AB_2138147 |
| Myc (mouse monoclonal, clone 9E10, used 1:5,000 in WB, 1:100 in ICC) | Santa Cruz Biotechnology | Cat# sc-40; RRID:AB_627268 |
| Peroxidase-conjugated donkey anti-rabbit IgG antibody | GE Healthcare | Cat# NA934; RRID:AB_772206 |
| Peroxidase-conjugated sheep anti-mouse IgG antibody | GE Healthcare | Cat# NA931; RRID:AB_772210 |
| Peroxidase-conjugated goat anti-chicken IgY antibody | Santa Cruz Biotechnology | Cat# sc-2428; RRID:AB_650514 |
| SNX27 (rabbit polyclonal, used 1:1000 in WB) | Proteintech | Cat# 16329-1-APRRID:AB_10888628 |
| VPS26 (rabbit monoclonal, clone EPR13456, used 1:1000 in WB) | Abcam | Cat# ab181352; RRID:AB_2665924 |
| VPS35 (rabbit polyclonal, used 1:500 in WB) | Proteintech | Cat# 10236-1-AP; RRID:AB_2215216 |
| Chemicals, peptides, and recombinant proteins | ||
| Bicuculline methiodide | Abcam | Cat# ab120108 |
| Forskolin | Sigma | Cat# F3917 |
| Complete, EDTA-free protease inhibitor cocktail | Sigma-Aldrich | Cat# 11836170001 |
| Duolink in situ PLA probe anti-mouse PLUS | Sigma-Aldrich | Cat#DUO92001 |
| Duolink in situ detection reagents red | Sigma-Aldrich | Cat#DUO92008 |
| Glutathione agarose | Thermo Scientific | Cat# 16100 |
| KN-93 | Abcam | Cat# ab120980 |
| Lipofectamine 2000 | Thermo Scientific | Cat# 11668019 |
| Neutravidin agarose | Thermo Scientific | Cat# 29204 |
| Papain suspension | Worthington | Cat# LS003126 |
| Phorbol 12-myristate 13-acetate (PMA) | Sigma | Cat# P8139 |
| Phosphatase inhibitor cocktail | AG Scientific | Cat# P-1517 |
| Strychnine hydrochloride | Sigma | Cat# S8753 |
| Sulfo-NHS-SS-biotin | CovaChem | Cat# 14207-100 |
| Tetrodotoxin citrate | Abcam | Cat# ab120055 |
| Experimental models: Cell lines | ||
| Human: HEK293T | ATCC | Cat# CRL-3216; RRID:CVCL_0063 |
| Experimental models: Organisms/strains | ||
| Rat: Sprague-Dawley | University of Queensland Biological Resources |
RRID:RGD_70508 |
| Recombinant DNA | ||
| FG12 | Qin et al., 2003 | Addgene plasmid #14884 |
| FG12-SNX27-shRNA | This paper | N/A |
| FUW-SNX27 WT | This paper | N/A |
| pCAG-HA-neuroligin-1B | Chih et al., 2006 | Addgene plasmid #15261 |
| pCIS | A gift from R. Huganir (Johns Hopkins University) | N/A |
| pCIS-GluN2A-Ctail (1213-1464) WT | This paper | N/A |
| pCIS-GluN2A-Ctail (1213-1464) S1459A | This paper | N/A |
| pCIS-GluN2A-Ctail (1213-1464) S1459E | This paper | N/A |
| pCIS-GluN2A-Ctail (1213-1464) V1464E | This paper | N/A |
| pCIS-GluN2A-Ctail (1364-1464) WT | This paper | N/A |
| pCIS-GluN2A-Ctail (1364-1464) S1459A | This paper | N/A |
| pCIS-GluN2A-Ctail (1364-1464) S1459D | This paper | N/A |
| pCIS-GluN2A-Ctail (1364-1464) V1464E | This paper | N/A |
| pCIS-SNX27 WT | This paper | N/A |
| pEGFP-C1 | Clontech | N/A |
| pEGFP-SNX27 WT | Clairfeuille et al., 2016 | N/A |
| pEGFP-truncated CaMKIIα | A gift from P. Opazo (University of Queensland) (Opazo et al., 2010) | N/A |
| pGEX4T-2-SNX27 PDZ domain (38-135) | Clairfeuille et al., 2016 | N/A |
| pLentiCRISPR-GFP | A gift from S. Incontro (University of California, San Francisco) (Incontro et al., 2018) | N/A |
| pLentiCRISPR-CaMKIIα-sgRNA (rat specific) | This paper | N/A |
| pMD2.G | A gift from D. Trono (Swiss Federal Institute of Technology Lausanne) | Addgene plasmid #12259 |
| pMDLg/pRRE | A gift from D. Trono (Swiss Federal Institute of Technology Lausanne) | Addgene plasmid #12251 |
| pRK5-GluN1 | Chen et al., 2020 | N/A |
| pRK5-SEP-GluN2A WT | This paper | N/A |
| pRK5-SEP-GluN2A S1459A | This paper | N/A |
| pRK5-SEP-GluN2A S1459D | This paper | N/A |
| pRK5-SEP-GluN2A S1459G | This paper | N/A |
| pRK5-myc-SNX27 WT | Hussain et al., 2014 | N/A |
| pRK5-myc-SNX27 L65A | This paper | N/A |
| pRK5-myc-SNX27 H112A | This paper | N/A |
| pRSV-Rev | A gift from D. Trono (Swiss Federal Institute of Technology Lausanne) | Addgene plasmid #12253 |
| Software and algorithms | ||
| Fiji | National Institutes of Health | RRID:SCR_002285; https://fiji.sc |
| Image Studio Lite | LI-COR | RRID:SCR_015795; https://www.licor.com/bio/image-studio-lite/ |
| GraphPad Prism 9 | GraphPad | RRID:SCR_002798; https://www.graphpad.com/ |
| pClamp10 | Molecular Devices | RRID:SCR_011323; https://www.moleculardevices.com/products/software/pclamp.html |
| QuB software | Nicolai and Sachs, 2013 | RRID:SCR_018076; https://qub.mandelics.com/ |