Figure S4.

dMMR CRCs express more of select chemokines and chemokine receptors than CIN CRCs but similar levels of TGFβ-associated genes. (A–C) Expression of genes associated with the “TGFβ signaling” GO term gene (A), chemokines (B), and chemokine receptors (C) in orthotopic CRC cells. (D) Induction of CD103 on CD8⁺ T cells by dMMR and CIN CRCs was measured by flow cytometry after coculturing cells directly or separated by a 0.4-µm Transwell filter for 24 h. CRC cells were pretreated for 24 h by 10 µg/ml anti-IFNAR1 or isotype control. Representative data from n = 3 repeats. dMMR versus CIN of same treatment condition: *, P ≤ 0.05; **, P ≤ 0.01. (E) Dependence of chemokine endogenous signaling on cGAS/STING, STAT1, or STAT3 was assessed by treatment of CRC cells for 1 h with 10 µM CCCP, 10 µM fludarabine (iSTAT1), or 50 µM S3I-201 (iSTAT3), respectively. n = 3 repeats.