Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 26;220(9):e202101075. doi: 10.1083/jcb.202101075

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Song et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Figure S2. — Neuronal differentiation of N2A cells and mESCs.(A) Cellular morphology of N2A cells cultured in low-serum medium with RA (10 µM) for 3 and 6 d. Scale bars, 50 µm. (B) Immunoblots of Ngn2 and actin in N2A cells untreated and cultured in RA (10 µM) for 3 and 6 d. (C) Cellular morphology of undifferentiated pluripotent ESCs (ES-D0), EBs of ES differentiated 8 d in KSR medium (ES-D8), and EBs trypsinized in N2 medium for an additional 4 d (ES-D12). Immunostaining of ES-D12 neuronal cells with Tuj1 (red, Alexa Fluor 568) and DAPI (blue). Scale bars, 200 µm. (D) The expression of pluripotent markers Oct4 and Nanog, neural progenitor markers Nestin and Sox1, and neuronal markers Tuj1 and Map2 was determined by RT-PCR. Samples were collected as described in C. Data plotted are from two independent experiments, each with triplicate qPCR reactions; error bars represent SD from independent samples.