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. Author manuscript; available in PMC: 2022 Jan 27.
Published in final edited form as: J Am Chem Soc. 2021 Jan 12;143(3):1296–1300. doi: 10.1021/jacs.0c12044

Figure 1.

Figure 1

A) Agarose gel (2%) electrophoresis of the conjugation reaction mixture (lane 1) between N3-PR10 and DBCO-ASO, purified ASO-PR10 conjugate (lane 2), and free ASO (lane 3). B) TEM image of the ASO-PR10 nanoparticles. Samples were stained with uranyl acetate (2%). C,D) Flow cytometry measurement of B16F10 cells treated with Cy5-labeled ASO-PR10 SNAs or Cy5-labeled free ASO (0.2-2 μM ASO, 4 h), showing significantly (>100×) higher uptake for the SNAs. Error bar: mean ± SD; n = 3. E) Confocal microscopy imaging of cells treated with 2 μM of Cy5-labeled ASO-PR10 nanoparticles or free ASO. Images taken with identical settings. Scale bar: 20 μm.