Figure 3.
Comparison of the stress tolerance ability of strains to oxidative stress and cell wall-perturbing agents
(A) Colony morphologies of the WT and mutant strains under oxidative stress conditions and in the presence of cell wall-perturbing agents.
(B–E) Colony diameters and the relative growth inhibition (RGI) values of the strains cultured in the presence of (B) 5–15 mM H2O2, (C) 0.01–0.05 mM menadione, (D) 0.01–0.03% SDS, (E) 20–100 ng/mL Congo red.
(F) Relative transcription levels (RTLs) of oxidation-related genes in the mutants when compared with the WT strain at different time points.
(G) RTLs of cell wall synthesis-related genes in the mutants when compared with the WT strain at different time points. The red line indicates the standard (which has an RTL of 1) for the statistical analysis of the RTL of each gene in a deletion mutant compared with that in the WT strain under a given condition. Error bars in (B–G): Data are represented as mean ± SD. The asterisk in (B–G) indicates a significant difference between the mutants and the WT strain (n = 3 for the WT strain (B–E), n = 9 for each mutant strain (B–E), n = 3 for each gene (F, G); Tukey's HSD, p < 0.05).