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. 2021 Jul 8;24(8):102820. doi: 10.1016/j.isci.2021.102820

Figure 4.

Figure 4

Comparison of trap formation, nematicidal activity, and extracellular proteolytic activity in the WT and mutant strains

(A) Trap formation in the WT and mutant strains induced by nematodes at different time points. The red arrows point toward the traps produced by the WT strain and mutants. Scale bar, 100 μm.

(B) The numbers of traps produced by the WT and mutant strains.

(C) The percentage of captured nematodes at different time points.

(D) Comparison of the extracellular proteolytic activities on casein plates.

(E) Total extracellular protease activity of the WT and mutant strains exhibited on the 7-day-old PD broth.

(F) Relative transcription levels (RTLs) of the genes encoding serine proteases in the mutants when compared with the WT strain at different time points. The red line indicates the standard (which has an RTL of 1) for the statistical analysis of the RTL of each gene in a deletion mutant compared with that in the WT strain under a given condition. Error bars in (B, C, E, F): Data are represented as mean ± SD. The asterisk in (B, C, E, F) indicates a significant difference between the mutants and the WT strain (n = 3 for the WT strain (B, C, E), n = 9 for each mutant strain (B, C, E), n = 3 for each gene (F); Tukey's HSD, p < 0.05).