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. 2021 Jul 8;24(8):102820. doi: 10.1016/j.isci.2021.102820

Figure 7.

Figure 7

Effect of AoRas2 and AoRheb on mitochondrial morphology and activity

(A) Mitochondrial morphology and mitochondrial membrane potential (MMP) of mycelia were compared between the WT and mutant strains. a. Mycelia were stained with MitoTracker Green. b–d. MMP was analyzed through tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) staining. With JC-1 staining, normal mitochondria mainly yield red fluorescence due to high MMP, whereas abnormal mitochondria mainly yield green fluorescence due to low MMP.

(B) Mitochondrial morphology and MMP of conidia were compared between the WT and mutant strains. a. Spores were stained with MitoTracker Red CMXRos, which can specifically label bioactive mitochondria. b–d. MMP was analyzed through JC-1 staining. Samples were examined under a confocal laser scanning microscope. Scale bar, 10 μm.

(C and D) MMP level analysis in (C) mycelia and (D) conidia. The ratio of red to green fluorescence intensity was obtained for at least 30 fields viewed under a microscope, and the horizontal bars depict the median. The asterisk in (C and D) indicates a significant difference between the mutants and the WT strain (Tukey's HSD, p < 0.05).