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. 2021 Jul 26;4:913. doi: 10.1038/s42003-021-02438-x

Fig. 1. Btnl2 is preferentially expressed in small intestinal epithelial cells.

Fig. 1

a Schematic representation of the WT and targeted locus of Btnl2−/− mice. hUb, human Ubiquitin promoter. b Beta-galactosidase and Neutral Red counterstaining in cryosections of segments of the small intestine (duodenum, jejunum, and ileum) and colon of 15-week-old Btnl2-KO mice. Arrowheads indicate regions of duodenal glands and duodenal, jejunal, ileal, and colonic crypts and villi with weak Btnl2-LacZ expression. Magnification is 20×; scale bar is 50 μm. c mRNA expression of Btnl2 in intestinal epithelial cells from different segments of small intestine and colon of cohoused 7-week-old Btnl2-KO and WT mice (n = 5, each), normalized to β2m. Error bars represent mean ± SEM. Significance is measured using multiple unpaired t-tests assuming similar SD, *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001, significantly different from WT mice.