Figure 2.

Patterns of viral shedding via the oropharyngeal (OP) (A) and cloacal (CL) (B) routes in SPF chickens. Groups of eight SPF chickens were inoculated intranasally with 10^6.0 EID₅₀ of each tested virus (LBM261/20 or LBM294/18). Three inoculated birds were euthanized at 3 dpi. At 1, 3, 5, 7, 10, and 14 dpi, OP and CL swabs were taken and subjected to rRT-PCR. The Ct value was converted to viral load using standard curves. Each bar represents the mean and standard deviation of 2–8 birds. ***; P < 0.0005, unpaired t test.