Table 3.

List of RAPD and ISSR primers’ names, sequences, annealing temperatures, bands sizes range, and number of bands amplified including monomorphic (MB) and polymorphic bands (PB)

Molecular marker	Primer name	Primer sequence 5′ to 3′	Temp. (°C)	Size range (bp)	Total amplified bands	MB	PB
RAPD	OPA-03	AGTCAGCCAC	39.5	1787–921	4	4	0
	OPA-04	AATCGGGCTG	39.5	2667–408	13	12	1
	OPH-07	CTGCATCGTG	39.5	2200–613	6	6	0
	OPI-07	CAGCGACAAG	39.5	1926–617	4	3	1
	OPK-07	AGCGAGCAAG	39.5	1788–690	11	11	0
	OPM-07	CCGTGACTCA	39.5	3860–840	7	7	0
	OPO-07	CAGCACTGAC	39.5	1642–538	4	4	0
	OPS-07	TCCGATGCTG	39.5	1813–516	4	4	0
	OPW-07	CTGGACGTCA	39.5	2114–625	6	6	0
	OPY-07	AGAGCCGTCA	39.5	1919–621	4	4	0
	Total				63	61	2
	Range			3860–408	13–4	12–4	2–0
	Average				6.3	6.1	0.2
ISSR	UBC-842C	GAGAGAGAGAGAGAGACG	59.9	2013–510	7	7	0
	UBC-842T	GAGAGAGAGAGAGAGATG	57.6	1617–567	9	7	2
	UBC-846A	CACACACACACACACACAAT	55.3	1717–520	4	3	1
	UBC-849C	GTGTGTGTGTGTGTGTCA	57.6	2682–678	13	12	1
	R(CA)7	GCACACACACACACA	53.4	1410–580	8	8	0
	UBC-834C	AGAGAGAGAGAGAGAGCT	57.6	4220–734	5	4	1
	UBC-834T	AGAGAGAGAGAGAGAGTT	55.3	1111–464	3	3	0
	UBC-848A	CACACACACACACACACAAG	57.6	1450–687	3	3	0
	UBC-860A	TGTGTGTGTGTGTGTGAA	55.3	2170–730	5	4	1
	(AC)8TG	ACACACACACACACACTC	57.6	2738–1540	9	8	1
	Total				66	59	7
	Range			4220–464	13–3	12–3	1–0
	Average				6.6	5.9	0.7