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. Author manuscript; available in PMC: 2022 Mar 5.
Published in final edited form as: Circ Res. 2021 Jan 27;128(5):570–584. doi: 10.1161/CIRCRESAHA.120.318511

Figure 3: Influenza infection induces cardiac proteome remodeling.

Figure 3:

Male and female 6-to-8-week-old wild-type and MLKL KO C57Bl/6 mice were intranasally infected with A/California/7/2009 (IAV) or mock challenged with vehicle (PBS) and hearts excised at day 12 post-infection. (A) Proteomic changes of mice hearts after IAV infection or mock challenge (n = 3 per condition). Hierarchical clustering of LFQ intensities of 295 significantly changed proteins (ANOVA, FDR < 0.01) revealed seven distinct clusters. Their abundance profiles among the groups were plotted in the middle panel. Enriched GO biological process terms are indicated for each marked cluster on the right panel. (B) Immunoblots for complement C3 (C3), AIAT, Catalase, HSP90, GST, GAPDH and VDAC and histograms of protein level quantification (n = 3 per group). Proteomic data of mice is representative from 2 separate experiments done with 3 mice of each sex; no sex based differences were observed. Kruskal-Wallis test with Dunn’s multiple-comparison post-test. Asterisks denote the level of significance observed: * = p ≤ 0.05; ** = p ≤ 0.01; *** = p ≤ 0.001.