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. 2021 Jul 27;7:55. doi: 10.1038/s41421-021-00284-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Size-exclusion chromatography (SEC) profile of purified WT hABCB6 reconstituted into lipid (POPC:POPE = 3:1) nanodiscs. UV absorbance (mAu), absorbance at 280 nm. Inset: SDS-PAGE analysis showing bands for hABCB6 (theoretical molecular weight (MW), 94 kDa) and the nanodisc scaffold protein MSP2N2 (theoretical MW, 30 kDa). b ATPase activity of hABCB6 in a lipid environment (blue squares, POPC and POPE nanodiscs; the blue curve is a fit to Michaelis–Menten kinetics) and in detergent (red circles and red curve, detergent C₁₂E₉). c Topology diagram of monomer hABCB6 with TMs and NBDs numbered. d Surface representation of apo-state hABCB6 in bottom and side views. e Cartoon representation of our apo-state hABCB6 model.