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. 2021 Jul 27;7:55. doi: 10.1038/s41421-021-00284-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Cavity 2 in occluded conformation. b Cavity 2 in inward-facing conformations. c The entrance of cavity 2 in inward-facing and occluded conformation. d The total expression levels of the wild-type, T320R, and plug-truncated form (residues 317–324 (GGGTGSTG) deleted) in HEK293F cells with actin as the loading control. The hABCB6 proteins carry a strep tag. e ATPase activity of hABCB6-WT, T320A, S322A, and T323A mutants with 4 mM ATP in the presence of 35 mM GSH or 10 μM PPIX. Error bars represent SD, n = 5. f ATPase activity of A-plug with 4 mM ATP in the presence of 35 mM GSH or 10 μM PPIX. Error bars represent SD, n = 5. g ATPase activity of W546A mutant with 4 mM ATP in the presence of 35 mM GSH or 10 μM PPIX. Error bars represent SD, n = 5.