Fig. 1. C5aR1⁺ neutrophils accumulate in breast cancer with disease progression and poor patient survival.

A t-Distributed stochastic neighbor embedding plot of single-cell RNA-seq data from GSE114727. Identified clusters are represented by different colors (left). Violin plots showing expression of the indicated transcripts in each different cell type (right). B C5aR1⁺ neutrophil percentage in total CD66b⁺ neutrophils in peripheral blood from healthy donors or BC patients (top). Representative image of C5aR1 staining in CD66b⁺ neutrophils (bottom). C C5aR1⁺ neutrophil percentage in CD66b⁺ neutrophils in each tissue of patients with BC (left). Representative image of C5aR1 staining in CD66b⁺ neutrophils (bottom) (right). D The correlation between C5aR1⁺ neutrophil gene signature (CD66b, CD15, and C5aR1) and CD54 in human tumors was analyzed in TCGA. E Kaplan–Meier survival analysis showing overall survival and relapse-free survival based on the expression of C5aR1⁺ neutrophil gene signature in GSE20685 and GSE7390. ***P < 0.001.