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. 2021 Jul 26;12(8):738. doi: 10.1038/s41419-021-04035-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — A Interaction between NPM1 and three truncated GST-HO-1 was tested by GST pulldown assay. n = 4. Detection of truncated HO-1 fragments with a Flag tag using (B) anti-Flag antibody; (C) anti-HO-1_aa1-30 (Abcam, ab13248, corresponding to amino acids 1-30 of HO-1); and (D) anti-HO-1_C-terminus (Abcam, ab52947, corresponding to C-terminus of HO-1). Red arrow indicates the fragments of HO-1. n = 3. E, F Transfection with HO-1_131-266 or HO-1_1-266 instead of HO-1_1-65 and HO-1_66-130 reversed the increasing fluorescence signal of nucleoplasmic NPM1 and the decreasing p53-MDM2 interaction induced by H₂O₂. ^*P < 0.05 vs. Vector; ^#P < 0.05 vs. Vector+H₂O₂; and n.s. (non significant) vs. Vector+H₂O₂. n = 3. G Transfection with HO-1_131-266 or HO-1_1-266 instead of HO-1_1-65 and HO-1_66-130 reversed the increasing proportion of SA-β-gal-positive cells induced by H₂O₂. ^*P < 0.05 vs. Vector; ^#P < 0.05 vs. Vector+H₂O₂; and n.s. vs. Vector+H₂O₂. n = 5.