Wash Buffer | Final concentration | Amount |
---|---|---|
1M Hepes-KOH, pH 7.6 | 50 mM | 12.5 mL |
5M LiCl | 500 mM | 25 mL |
0.5M EDTA | 1 mM | 0.5 mL |
10% NP-40 | 1% | 25 mL |
10% Na-Deoxycholate | 0.7% | 17.5 mL |
ddH2O | n/a | 169.5 mL |
Total | n/a | 250 mL |
Wash Buffer | Final concentration | Amount |
---|---|---|
1M Hepes-KOH, pH 7.6 | 50 mM | 12.5 mL |
5M LiCl | 500 mM | 25 mL |
0.5M EDTA | 1 mM | 0.5 mL |
10% NP-40 | 1% | 25 mL |
10% Na-Deoxycholate | 0.7% | 17.5 mL |
ddH2O | n/a | 169.5 mL |
Total | n/a | 250 mL |