Elution Buffer | Final concentration | Amount |
---|---|---|
1M Tris-HCl, pH 8.0 | 50 mM | 5 mL |
0.5M EDTA | 10 mM | 2 mL |
10% SDS | 1% | 10 mL |
ddH2O | n/a | 83 mL |
Total | n/a | 100 mL |
Elution Buffer | Final concentration | Amount |
---|---|---|
1M Tris-HCl, pH 8.0 | 50 mM | 5 mL |
0.5M EDTA | 10 mM | 2 mL |
10% SDS | 1% | 10 mL |
ddH2O | n/a | 83 mL |
Total | n/a | 100 mL |