LB3 | Final concentration | Amount |
---|---|---|
Tris-HCl, pH 8.0 | 10 mM | 1 mL |
5M NaCl | 100 mM | 2 mL |
0.5M EDTA | 1 mM | 0.2 mL |
0.5M EGTA | 0.5 mM | 0.1 mL |
10% Na-Deoxycholate | 0.1% | 1 mL |
20% N-lauroylsarcosine | 0.5% | 2.5 mL |
ddH2O | n/a | 93.2 mL |
Total | n/a | 100 mL |