Figure 1.

Representative images of different sized organoids, healthy and unhealthy organoids

Day1 after the splitting: the organoids are small in size (A), the size and number of the organoids are increased with time till they become medium sized (B) and large sized (C). (A–C) represent examples of healthy organoids. Upper panel is 4× images and lower panel is 10× images (D and E) represent examples of unhealthy organoids, arrows point to the organoids where the black spots are accumulated as a sign of cell death. Representative Images are 10×. Scale bar 100 μm